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We subsequently employed Seahorse analysis to investigate whether KL1 altered metabolic activity of extracellular S

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Epidermal penetration stage: Resistance peak 2.0 Newtons (accompanied by a slight barrier breakthrough sensation) Through the dermal reticular layer: Continuous resistance 1.5 Newtons (similar to the toughness of piercing rubber) Submerging into superficial fat: Resistance drops sharply to 0.2 Newtons (similar to the hollowness of sponge gaps) Touching muscle fascia: Resistance rises back to 1.8 Newtons (a firm and elastic contractile sensation) If during insertion, the resistance value suddenly drops below 0.1 Newtons and is accompanied by obvious pain feedback from the patient, the tip may have slipped into a tiny venous lumen 15 mm under the skin